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Nitroquinoline as an oral antibacterial drug for the treatment of urinary tract infections (2).jpg

Nitroquinoline as an oral antibacterial drug for the treatment of urinary tract infections (1).jpg

Nitroxoline: China Suppliers and Factory Offering Effective Oral Antibacterial for Urinary Tract Infections

Nitroxoline (5-nitroquinolin-8-ol) is a potent urinary antibacterial agent that effectively targets susceptible gram-positive and gram-negative bacteria commonly responsible for urinary tract infections. As a leading choice among suppliers in China, our factory ensures the highest quality standards for Nitroxoline, making it an essential product for healthcare professionals dealing with urinary infections. Trust our China-based factory for reliable and effective antimicrobial solutions to improve patient outcomes

Product Detail

Product Parameters

Product Name	Nitroxoline
CAS NO	4008-48-4
Structure
Molecular formula	C9H6N2O3
Purity	Over 99.5%
Other product names	5-Nitro-8-quinolinol, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, 5-nitroquinolin-8-ol
Molecular weight	190.16

In vitro activity

Using spectrophotometric fluorescence assay, Nitroxoline can significantly reduce the degradation of extracellular DQ collagen IV in all tested cancer cell lines. Nitroxoline can significantly reduce real-time monitoring of tumor cell invasion and decrease the invasive growth of multicellular tumor spheroids, which can be used as a three-dimensional vitreous model of tumor invasion. In vitro angiogenesis experiments, Nitroxoline can significantly reduce the formation of endothelial tubes.

In vivo activity

Nitroxoline significantly inhibited tumor growth, angiogenesis and metastasis in LPB fibrosarcoma and MMTV PyMT breast cancer mouse models.

Kinase experiment

In Vitro Kinase Assays : The potency of SP600125 towards kinases, including MPS1, JNK, and Aurora kinase A, is determined based on the specific measurement of radioactive phosphotransfer to the substrate. For each enzyme, the absolute Km values for ATP and the specific substrate are initially determined and each assay is then run at optimized [ATP] (2·αKm) and [substrate] (5·Km) concentrations. MPS1 activity is measured using 5 nM of MPS1 recombinant protein in 50 mM HEPES pH 7.5, 2.5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, 3 μM NaVO3, 2 mM β-glycerophosphate, 0.2 mg/mL BSA, 200 μM P38-βtide substrate-peptide (KRQADEEMTGYVATRWYRAE), and 8 μM ATP with 1.5 nM 33P-γ-ATP. Ten serial 1:3 dilutions (from 30 μM to 1.5 nM) of SP600125 are tested and IC50 determined.

Cell experiment

Cell suspension are seeded in the wells of an E-plate 16 according to the manufacturer's instructions. After seeding, the CI is monitored every 15 min. After ∼10 h (MCF-10A neoT and MMTV-PyMT), 14 h (U-87 MG) or 24 h (LPB), when the cells are in their log phase of growth, 50 μl of the compound or 0.1% DMSO is added, and the experiment allowed to run for 72 h. Once every 24 h the medium is replaced with fresh medium containing the inhibitor or suitable control to prevent cell death due to medium depletion. Compounds and their concentrations are: nitroxoline (5 μM) and CA-074 (5 μM) for all cell lines other than MCF-10A neoT cell line, where nitroxoline was used at 2.5 μM. All measurements were performed in quadruplicate. (Only for Reference)

Frequently Asked Questions (FAQ)

What is the CAS number and molecular formula of Nitroxoline?

Nitroxoline has a CAS Registry Number of 4008-48-4 and a molecular formula of C9H6N2O3.

What are the alternative chemical names for Nitroxoline?

Nitroxoline is also known as 5-Nitro-8-quinolinol, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, and 5-nitroquinolin-8-ol.

How does Nitroxoline perform in in vitro activity assays?

In vitro, Nitroxoline significantly reduces the degradation of extracellular DQ collagen IV in cancer cell lines, decreases the invasive growth of multicellular tumor spheroids, reduces tumor cell invasion, and inhibits endothelial tube formation in angiogenesis experiments.

What effect does Nitroxoline have on tumor growth in vivo?

Nitroxoline has been shown to significantly inhibit tumor growth, angiogenesis, and metastasis in both LPB fibrosarcoma and MMTV PyMT breast cancer mouse models.

What concentrations of Nitroxoline are typically used in cell experiments?

In standard cell experiments, Nitroxoline is typically used at a concentration of 5 μM for most cell lines, and at 2.5 μM specifically for the MCF-10A neoT cell line.