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Nitroquinoline as an oral antibacterial drug for the treatment of urinary tract infections (2).jpg

Nitroquinoline as an oral antibacterial drug for the treatment of urinary tract infections (1).jpg

Nitroxoline: Effective Oral Antibacterial for UTIs - China Suppliers & Factory

Nitroxoline, also known as 5-nitroquinolin-8-ol, is a potent urinary antibacterial agent that effectively targets both gram-positive and gram-negative bacteria commonly associated with urinary tract infections. As a leading supplier in China, our factory is committed to producing high-quality Nitroxoline to meet the needs of healthcare providers and patients. With its proven efficacy, our product is an essential choice for those seeking reliable treatment options for urinary tract infections. Trust our China-based suppliers for top-grade Nitroxoline that ensures optimal health outcomes

Product Detail

Product Parameters

Product Name	Nitroxoline
CAS NO	4008-48-4
Structure
Molecular Formula	C9H6N2O3
Purity	Over 99.5%
Other Product Names	5-Nitro-8-quinolinol, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, 5-nitroquinolin-8-ol
Molecular Weight	190.16

In Vitro Activity

Using spectrophotometric fluorescence assay, Nitroxoline can significantly reduce the degradation of extracellular DQ collagen IV in all tested cancer cell lines. Nitroxoline can significantly reduce real-time monitoring of tumor cell invasion and decrease the invasive growth of multicellular tumor spheroids, which can be used as a three-dimensional vitreous model of tumor invasion. In vitro angiogenesis experiments, Nitroxoline can significantly reduce the formation of endothelial tubes.

In Vivo Activity

Nitroxoline significantly inhibited tumor growth, angiogenesis and metastasis in LPB fibrosarcoma and MMTV PyMT breast cancer mouse models.

Kinase Experiment

In Vitro Kinase Assays : The potency of SP600125 towards kinases, including MPS1, JNK, and Aurora kinase A, is determined based on the specific measurement of radioactive phosphotransfer to the substrate. For each enzyme, the absolute Km values for ATP and the specific substrate are initially determined and each assay is then run at optimized [ATP] (2·αKm) and [substrate] (5·Km) concentrations. MPS1 activity is measured using 5 nM of MPS1 recombinant protein in 50 mM HEPES pH 7.5, 2.5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, 3 μM NaVO3, 2 mM β-glycerophosphate, 0.2 mg/mL BSA, 200 μM P38-βtide substrate-peptide (KRQADEEMTGYVATRWYRAE), and 8 μM ATP with 1.5 nM 33P-γ-ATP. Ten serial 1:3 dilutions (from 30 μM to 1.5 nM) of SP600125 are tested and IC50 determined.

Cell Experiment

Cell suspension are seeded in the wells of an E-plate 16 according to the manufacturer's instructions. After seeding, the CI is monitored every 15 min. After ∼10 h (MCF-10A neoT and MMTV-PyMT), 14 h (U-87 MG) or 24 h (LPB), when the cells are in their log phase of growth, 50 μl of the compound or 0.1% DMSO is added, and the experiment allowed to run for 72 h. Once every 24 h the medium is replaced with fresh medium containing the inhibitor or suitable control to prevent cell death due to medium depletion. Compounds and their concentrations are: nitroxoline (5 μM) and CA-074 (5 μM) for all cell lines other than MCF-10A neoT cell line, where nitroxoline was used at 2.5 μM. All measurements were performed in quadruplicate. (Only for Reference)

Frequently Asked Questions (FAQ)

What is the CAS number and molecular weight of Nitroxoline?

Nitroxoline has a CAS number of 4008-48-4 and a molecular weight of 190.16.

What is the purity standard of this Nitroxoline product?

The purity level of this Nitroxoline product is over 99.5%.

What are some alternative chemical names for Nitroxoline?

Nitroxoline is also known as 5-Nitro-8-quinolinol, 8-Hydroxy-5-nitroquinoline, 5-Nitro-8-hydroxyquinoline, and 5-nitroquinolin-8-ol.

How does Nitroxoline function during in vitro activities?

In vitro, Nitroxoline significantly reduces the degradation of extracellular DQ collagen IV in cancer cell lines, decreases the invasive growth of multicellular tumor spheroids, and reduces endothelial tube formation during angiogenesis.

Has Nitroxoline been tested in animal models (in vivo)?

Yes. Nitroxoline has shown significant inhibition of tumor growth, angiogenesis, and metastasis in both LPB fibrosarcoma and MMTV PyMT breast cancer mouse models.

Which cell lines are used to test Nitroxoline in cell experiments?

Cell experiments utilize MCF-10A neoT, MMTV-PyMT, U-87 MG, and LPB cell lines to monitor cell index (CI) changes and growth phases.